ANOMALOUS METAPLASTIC OSSIFICATION OF ANIONIC COLLAGEN COATED POLYPROPYLENE MESH AFTER IMPLANTATION IN THE ABDOMINAL WALL

²Sueli Suzigan, ¹Gilberto Goissis, ²Diderot Rodrigues Parreira, ²Helder Chaves, ²Kassim M. K. Hussain, ²Selma R. O. Raymundo.

¹ Depto. Quím. Fís. Molec., Inst. Quím. São Carlos (IQSC) - Universidade de São Paulo (USP). ² Faculdade de Medicina de São José do Rio Preto (FAMERP). SP - Brazil

 

Images: Table and Figures Legends

	(a)	(b)

 

 

 

 

 

 

 

(c)

Figure 1: Scanning electron micrograph:

a -    polypropylene mesh (1000X);
b -    polypropylene: anionic collagen (500x);
c -     polypropylene: anionic  collagen  (5000x)

 

Tissue response	Explants (Time in weeks)
	Polypropylene				Polypropylene:anionic collagen
	1	9	18		1	9	18
I. Ulcerated skin	(-)	(-)	3+/5		(-)	(-)	(-)
II. Granulation tissue	(-)	(-)	3+/5		(-)	(-)	(-)
III. Fibrosis	3+/5	3+/5	3+/5		4+/5	4+/5	4+/5
IV. Inflamm. (PI)b	(-)	2+/5	4+/5		3+/5	1+/5	1+/5
IV.1. Histiocytes	(-)	1+/5	2+/5		2+/5	1+/5	1+/5
IV.3. CGM (CE)	(-)	1+/5	2+/5		2+/5	1+/5	1+/5
V. Osteoid	(-)	(-)	1+/5 (PI)		(-)	(-)	(-)
VI. Osteoblasts	(-)	(-)	1+/5		(-)	(-)	1+/5
VII. Osteoclasts	(-)	(-)	(-)		(-)	(-)	1+/5
VII. Primary bone	(-)	(-)	(-)		3+/5	5+/5	2+/5
VII.1. Localization	(-)	(-)	(-)		PI	ETI	PI
VIII. Calcification	(-)	(-)	(-)		(-)	(-)	(-)

Table 1: a– evaluation after the established the scale 1+/5: minimal response; 5+/5: intense response; b - CGM(CE): multinucleated giant cells (foreign body reaction); PI: near the implant; ETI: surrounding all the implant; (-) absent.

 

 

 (a)                                                    (b)

 

  

 

 

(c)                                                       (d)

 

(e)

 

 

 

 

 

Figure 2: Histologic findings of the tissular response of polypropylene (PLP) and PLP:anionic collagen (AC) composite after 18 weeks from implantation. a – PLP, intense fibrosis around the implant (top center), Gomori´s trichrome stain, 40x; b – PLP:AC, associated to fibrosis and new bone formation (right), H.E., 40x; c – Primitive bone (center), close to PLP (left), H.E., 100x; d – Bone trabeculae with a lacunae at the central region, H.E., 400x; e -  Osteoblasts at the surface of the primary spicule (inner surface), H.E., 400x.